Format

Send to

Choose Destination
Biochim Biophys Acta. 2016 Nov;1861(11):1693-1704. doi: 10.1016/j.bbalip.2016.07.007. Epub 2016 Jul 22.

Circulating levels of endocannabinoids and oxylipins altered by dietary lipids in older women are likely associated with previously identified gene targets.

Author information

1
Department of Nutrition, University of California, Davis, Davis, CA, United States; Center on Aging, University of Connecticut Health Center, Farmington, CT, United States. Electronic address: baw@purdue.edu.
2
Genome and Biomedical Sciences Facility, University of California, Davis, Davis, CA, United States. Electronic address: jefkim@ucdavis.edu.
3
Center on Aging, University of Connecticut Health Center, Farmington, CT, United States. Electronic address: kenny@uchc.edu.
4
USDA, ARS, WHNRC, Davis, CA, United States. Electronic address: Theresa.Pedersen@ARS.USDA.GOV.
5
Metabolon, Inc., Durham, NC, United States. Electronic address: KPappan@metabolon.com.
6
Department of Nutrition, University of California, Davis, Davis, CA, United States; USDA, ARS, WHNRC, Davis, CA, United States. Electronic address: John.Newman@ARS.USDA.GOV.

Abstract

Postmenopausal women (PMW) report marginal n-3 PUFA intakes and are at risk of chronic diseases associated with the skeletal, muscular, neuroendocrine, and cardiovascular systems. How n-3 PUFA affect the amounts of endocannabinoids (ECs) and oxylipins (OLs) of metabolic and physiologic importance in PMW is not clear. Based on our recent findings that dietary n-3 PUFA alter gene targets of the EC system and lower pro-inflammatory OL we proceeded to characterize these actions in blood of PMW. Our aim was to determine levels of the ECs, OLs, and global metabolites (GM) in white PMW (75±7y), randomized in a double-masked manner, from baseline to 6mo after receiving a fish oil supplement of n-3 PUFA (720mg 20:5n3+480mg 22:6n3/d, n=20) or placebo (1.8g oleic acid/d, n=20). ECs and OLs in serum were determined by UPLC-MS/MS and GM by GC-MS and LC-MS/MS. Plasma 20:5n3 and 22:6n3 levels increased in PMW given fish oil. EC n-6 acyl-ethanolamides, arachidonate-derived diols were decreased and 20:5n3 and 22:6n3 diols, epoxides, and alcohols were increased in PMW given fish oil. GM analysis revealed that n-3 PUFA supplementation increased renal steroid hormone and proteolytic metabolite levels in PMW. Herein, we confirm that gene targets of the EC system, previously found as modifiable by n-3 PUFA result in changes in the levels of ECs and OLs in PMW. This study shows phenotypic responses (in levels) to n-3 PUFA supplementation in PMW and increases of n-3 acyl-ethanolamide and n-3-derived OL of clinical considerations in aging.

KEYWORDS:

Endocannabinoids; Global metabolites; Oxylipins; Postmenopausal women

PMID:
27452639
DOI:
10.1016/j.bbalip.2016.07.007
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center