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Trends Genet. 2016 Sep;32(9):566-575. doi: 10.1016/j.tig.2016.06.007. Epub 2016 Jul 19.

Regulation of Single-Strand Annealing and its Role in Genome Maintenance.

Author information

1
Department of Cancer Genetics and Epigenetics, Beckman Research Institute of the City of Hope, Duarte, CA, USA; Irell and Manella Graduate School of Biological Sciences, Beckman Research Institute of the City of Hope, Duarte, CA, USA.
2
Department of Cancer Genetics and Epigenetics, Beckman Research Institute of the City of Hope, Duarte, CA, USA.
3
Department of Cancer Genetics and Epigenetics, Beckman Research Institute of the City of Hope, Duarte, CA, USA; Irell and Manella Graduate School of Biological Sciences, Beckman Research Institute of the City of Hope, Duarte, CA, USA. Electronic address: jstark@coh.org.

Abstract

Single-strand annealing (SSA) is a DNA double-strand break (DSB) repair pathway that uses homologous repeats to bridge DSB ends. SSA involving repeats that flank a single DSB causes a deletion rearrangement between the repeats, and hence is relatively mutagenic. Nevertheless, this pathway is conserved, in that SSA events have been found in several organisms. In this review, we describe the mechanism of SSA and its regulation, including the cellular conditions that may favor SSA versus other DSB repair events. We will also evaluate the potential contribution of SSA to cancer-associated genome rearrangements, and to DSB-induced gene targeting.

KEYWORDS:

RAD51; RAD52; alternative end joining; end resection; homology directed repair; single-strand annealing

PMID:
27450436
PMCID:
PMC4992407
DOI:
10.1016/j.tig.2016.06.007
[Indexed for MEDLINE]
Free PMC Article

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