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Biochemistry (Mosc). 2016 Jul;81(7):755-61. doi: 10.1134/S0006297916070129.

Peptide Aβ(16-25) Forms Nanofilms in the Process of Its Aggregation.

Author information

1
Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia. ogalzit@vega.protres.ru.

Abstract

A method for the synthesis and high purification of fragments of Aβ(1-42) peptide has been elaborated. We have synthesized the amyloidogenic fragment Aβ(16-25) predicted by us and studied the process of its aggregation by electron microscopy and X-ray analysis. Electron microscopy images show that the peptide forms a film, which is not characteristic of amyloid fibrils. At the same time, according to the X-ray diffraction data, its preparations display the presence of two main reflections (4.6-4.8 and 8-12 Å) characteristic of cross-β structure of amyloid fibrils. Thus, the fragment Aβ(16-25) that we predicted is a promising object not only for studying the process of polymerization of the peptides/proteins, but also for using it as a nanomaterial to study a number of biological processes.

PMID:
27449622
DOI:
10.1134/S0006297916070129
[Indexed for MEDLINE]
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