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J Med Virol. 2017 Feb;89(2):324-331. doi: 10.1002/jmv.24639. Epub 2016 Sep 7.

Functional impairment of CMV-reactive cellular immunity during pregnancy.

Author information

1
Department of Obstetrics and Gynecology, University of Regensburg, Hospital of the Barmherzige Brueder, Regensburg, Germany.
2
Lophius Biosciences GmbH, Regensburg, Germany.
3
Center for Clinical Studies, University of Regensburg, Regensburg, Germany.

Abstract

Cytomegalovirus (CMV) is the most common congenital viral infection. Mother-to-child transmission can cause severe child disability. Intact CMV-specific cell-mediated immunity (CMI) was shown to prevent uncontrolled replication in healthy individuals. This study aimed to determine whether CMV-specific CMI is impaired in pregnant women, thus potentially increasing the overall risk for active CMV replication and transmission. CMV-specific CMI in peripheral blood of 60 pregnant women was determined using T-Track® CMV for detection of IE-1 and pp65-reactive effector cells by IFN-γ ELISpot, and compared to the CMV-IgG and -IgM serostatus. CMV-specific CMI was detected in 65% of CMV-seropositive pregnant women. Five percent of CMV-IgG seronegative women showed IE-1- but not pp65-reactive cells. The overall number of CMV-reactive cells in pregnant women was significantly lower compared to a matched non-pregnant control group (P < 0.001). No significant difference in CMV-specific CMI was detected in the course of the three trimesters of pregnancy of CMV-IgG seropositive women. Postpartum (median days postnatal = 123), IE-1- and pp65-specific CMI remained significantly lower than in the non-pregnant control group (P < 0.001 and 0.0032, respectively). Functional analysis of CMV-reactive immune cells using T-Track® CMV therefore suggests a systemic down-regulation of CMV-specific CMI in pregnant women. Further studies are needed to investigate whether this may be indicative of a higher susceptibility to CMV reactivation or transmission. J. Med. Virol. 89:324-331, 2017.

KEYWORDS:

cell-mediated immunity; cytomegalovirus; interferon (IFN)-γ-enzyme-assay ELISpot; pregnancy

PMID:
27447923
DOI:
10.1002/jmv.24639
[Indexed for MEDLINE]

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