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Bio Protoc. 2014 Dec 5;4(23). pii: e1304.

Fluorescent Measurement of Synaptic Activity Using SynaptopHluorin in Isolated Hippocampal Neurons.

Author information

1
Department of Internal Medicine (Endocrinology), School of Medicine, Yale University, New Haven, USA.
2
Department of Internal Medicine (Endocrinology), School of Medicine, Yale University, New Haven, USA; Department of Neurobiology, School of Medicine, Yale University, New Haven, USA.

Abstract

This protocol comprises the entire process of fluorescent measurement of vesicle recycling using the probe SynaptopHluorin, a pH-dependent GFP variant whose fluorescence increases at the synapse upon vesicle release due to fluorescence quenching in acidic vesicles. This technique provides a genetic tool to monitor synaptic vesicle recycling in real time in cultured hippocampal neurons.

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