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Science. 2016 Aug 5;353(6299):598-602. doi: 10.1126/science.aaf8084. Epub 2016 Jul 21.

Spatial organization of chromatin domains and compartments in single chromosomes.

Author information

1
Howard Hughes Medical Institute, Department of Chemistry and Chemical Biology, Department of Physics, Harvard University, Cambridge, MA 02138, USA.
2
Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.
3
Department of Genetics, Harvard Medical School, Boston, MA 02115, USA. zhuang@chemistry.harvard.edu twu@genetics.med.harvard.edu.
4
Howard Hughes Medical Institute, Department of Chemistry and Chemical Biology, Department of Physics, Harvard University, Cambridge, MA 02138, USA. zhuang@chemistry.harvard.edu twu@genetics.med.harvard.edu.

Abstract

The spatial organization of chromatin critically affects genome function. Recent chromosome-conformation-capture studies have revealed topologically associating domains (TADs) as a conserved feature of chromatin organization, but how TADs are spatially organized in individual chromosomes remains unknown. Here, we developed an imaging method for mapping the spatial positions of numerous genomic regions along individual chromosomes and traced the positions of TADs in human interphase autosomes and X chromosomes. We observed that chromosome folding deviates from the ideal fractal-globule model at large length scales and that TADs are largely organized into two compartments spatially arranged in a polarized manner in individual chromosomes. Active and inactive X chromosomes adopt different folding and compartmentalization configurations. These results suggest that the spatial organization of chromatin domains can change in response to regulation.

PMID:
27445307
PMCID:
PMC4991974
DOI:
10.1126/science.aaf8084
[Indexed for MEDLINE]
Free PMC Article

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