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Gene. 1989 Apr 15;77(1):133-40.

Expression of a mouse metallothionein gene in transgenic plant tissues.

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Laboratoire de Biologie Cellulaire, INRA-Centre de Versailles, France.


Three gene constructions based on a mouse metallothionein I gene (mMT-I) were introduced into tobacco using a Ri plasmid vector system to test the effectiveness of animal gene regulatory signals in plant cells. No transcription from the native mouse gene was observed. In plant cells bearing chimeric mMT-I genes in which transcription was driven by the nopaline synthase promoter, neither polyadenylation nor splicing of mMT-I pre-mRNA was observed. Detailed comparisons of mMT-I sequences with those of known plant genes were carried out; slight differences in regions of known consensus sequences may be at least partly responsible for the non-recognition of mMT-I gene regulatory signals in plant cells, though other as yet unidentified, potentially necessary sequences may also be involved.

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