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PLoS One. 2016 Jul 21;11(7):e0159482. doi: 10.1371/journal.pone.0159482. eCollection 2016.

Feasibility of Human Amniotic Fluid Derived Stem Cells in Alleviation of Neuropathic Pain in Chronic Constrictive Injury Nerve Model.

Author information

1
Institute of Biomedical Sciences, National Chung-Hsing University, Taichung, Taiwan.
2
Faculty of Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan.
3
Department of Medical Research, Taichung Veterans General Hospital, Taichung, Taiwan.
4
Institute of Life Sciences, National Chung-Hsing University, Taichung, Taiwan.
5
Department of Neurosurgery, Taichung Veterans General Hospital, Taichung, Taiwan.

Abstract

PURPOSE:

The neurobehavior of neuropathic pain by chronic constriction injury (CCI) of sciatic nerve is very similar to that in humans, and it is accompanied by a profound local inflammation response. In this study, we assess the potentiality of human amniotic fluid derived mesenchymal stem cells (hAFMSCs) for alleviating the neuropathic pain in a chronic constriction nerve injury model.

METHODS AND METHODS:

This neuropathic pain animal model was conducted by four 3-0 chromic gut ligatures loosely ligated around the left sciatic nerve in Sprague-Dawley rats. The intravenous administration of hAFMSCs with 5x105 cells was conducted for three consecutive days.

RESULTS:

The expression IL-1β, TNF-α and synaptophysin in dorsal root ganglion cell culture was remarkably attenuated when co-cultured with hAFMSCs. The significant decrease of PGP 9.5 in the skin after CCI was restored by administration of hAFMSCs. Remarkably increased expression of CD 68 and TNF-α and decreased S-100 and neurofilament expression in injured nerve were rescued by hAFMSCs administration. Increases in synaptophysin and TNF-α over the dorsal root ganglion were attenuated by hAFMSCs. Significant expression of TNF-α and OX-42 over the dorsal spinal cord was substantially attenuated by hAFMSCs. The increased amplitude of sensory evoked potential as well as expression of synaptophysin and TNF-α expression was alleviated by hAFMSCs. Human AFMSCs significantly improved the threshold of mechanical allodynia and thermal hyperalgesia as well as various parameters of CatWalk XT gait analysis.

CONCLUSION:

Human AFMSCs administration could alleviate the neuropathic pain demonstrated in histomorphological alteration and neurobehavior possibly through the modulation of the inflammatory response.

PMID:
27441756
PMCID:
PMC4956194
DOI:
10.1371/journal.pone.0159482
[Indexed for MEDLINE]
Free PMC Article

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