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Pediatr Res. 2016 Nov;80(5):710-718. doi: 10.1038/pr.2016.149. Epub 2016 Jul 21.

Short-term effects of cannabidiol after global hypoxia-ischemia in newborn piglets.

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Department of Pediatric Research, Women and Children's Division and Institute for Surgical research, Oslo University Hospital, Rikshospitalet, Oslo, Norway.
University of Oslo, Oslo, Norway.
Neonatal Research Unit, Health Research Institute Hospital La Fe, Valencia, Spain.
Division of Neonatology. Hospital Clinico San carlos-IdISSC, Madrid, Spain.
Department of Pathology, Oslo University Hospital, Ullevål, Oslo, Norway.



Cannabidiol (CBD), a nonpsychoactive cannabinoid, has shown neuroprotective actions after neonatal hypoxia-ischemia (HI) in animals. We wanted to further explore the effects of CBD, alone and in conjunction with hypothermia, in a piglet model of global HI.


Fifty-five anesthetized newborn piglets were randomized to either controls (n = 7) or HI (n = 48) by ventilation with 8% O2 until mean arterial blood pressure reached 20 mmHg and/or base excess reached -20 mmol/l. After resuscitation piglets were randomized to either: vehicle (VEH), CBD 1mg/kg, VEH+hypothermia (H) or CBD 1mg/kg+H (each n = 12). Piglets were euthanized 9.5 h after HI and plasma, urine, cerebrospinal fluid, and brain tissue were sampled for analysis.


HI induced global damage with significantly increased neuropathology score, S100B in cerebrospinal fluid, hippocampal proton magnetic resonance spectroscopy biomarkers, plasma troponin-T, and urinary neutrophil gelatinase-associated lipocalin. CBD alone did not have any significant effects on these parameters while CBD+H reduced urinary neutrophil gelatinase-associated lipocalin compared with VEH+H (P < 0.05). Both hypothermic groups had significantly lower glutamate/N-acetylaspartate ratios (P < 0.01) and plasma troponin-T (P<0.05) levels compared with normothermic groups.


In contrast to previous studies, we do not find significant protective effects of CBD after HI in piglets. Evaluation of CBD in higher doses might be warranted.

[Indexed for MEDLINE]

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