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Plant Physiol. 2016 Sep;172(1):38-61. doi: 10.1104/pp.16.00354. Epub 2016 Jul 19.

Indel Group in Genomes (IGG) Molecular Genetic Markers.

Author information

1
Department of Biochemistry and Molecular Medicine (T.W.T.), Department of Plant Sciences (D.B.-W., T.H., R.C.), Department of Plant Biology (M.R., S.K., A.B., S.M.B.), and Genome Center (S.M.B.), University of California, Davis, California 95616.
2
Department of Biochemistry and Molecular Medicine (T.W.T.), Department of Plant Sciences (D.B.-W., T.H., R.C.), Department of Plant Biology (M.R., S.K., A.B., S.M.B.), and Genome Center (S.M.B.), University of California, Davis, California 95616 sbrady@ucdavis.edu.

Abstract

Genetic markers are essential when developing or working with genetically variable populations. Indel Group in Genomes (IGG) markers are primer pairs that amplify single-locus sequences that differ in size for two or more alleles. They are attractive for their ease of use for rapid genotyping and their codominant nature. Here, we describe a heuristic algorithm that uses a k-mer-based approach to search two or more genome sequences to locate polymorphic regions suitable for designing candidate IGG marker primers. As input to the IGG pipeline software, the user provides genome sequences and the desired amplicon sizes and size differences. Primer sequences flanking polymorphic insertions/deletions are produced as output. IGG marker files for three sets of genomes, Solanum lycopersicum/Solanum pennellii, Arabidopsis (Arabidopsis thaliana) Columbia-0/Landsberg erecta-0 accessions, and S. lycopersicum/S. pennellii/Solanum tuberosum (three-way polymorphic) are included.

PMID:
27436831
PMCID:
PMC5074621
DOI:
10.1104/pp.16.00354
[Indexed for MEDLINE]
Free PMC Article

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