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Anal Chem. 2016 Aug 16;88(16):8043-9. doi: 10.1021/acs.analchem.6b01373. Epub 2016 Jul 28.

Dynamic Modulation of DNA Hybridization Using Allosteric DNA Tetrahedral Nanostructures.

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Division of Physical Biology and Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences , Shanghai 201800, China.
School of Chemistry and Molecular Engineering, East China Normal University , Shanghai 200241, China.
Key Laboratory for Organic Electronics and Information Displays (KLOEID), Institute of Advanced Materials (IAM) and School of Materials Science and Engineering, Nanjing University of Posts and Telecommunications , Nanjing, 210046, China.
Chemistry Department, King Saud University , Riyadh 11451, Saudi Arabia.
Kellogg College, University of Oxford , Oxford, OX2 6PN, United Kingdom.


The fixed dynamic range of traditional biosensors limits their utility in several real applications. For example, viral load monitoring requires the dynamic range spans several orders of magnitude; whereas, monitoring of drugs requires extremely narrow dynamic range. To overcome this limitation, here, we devised tunable biosensing interface using allosteric DNA tetrahedral bioprobes to tune the dynamic range of DNA biosensors. Our strategy takes the advantage of the readily and flexible structure design and predictable geometric reconfiguration of DNA nanotechnology. We reconfigured the DNA tetrahedral bioprobes by inserting the effector sequence into the DNA tetrahedron, through which, the binding affinity of DNA tetrahedral bioprobes can be tuned. As a result, the detection limit of DNA biosensors can be programmably regulated. The dynamic range of DNA biosensors can be tuned (narrowed or extended) for up to 100-fold. Using the regulation of binding affinity, we realized the capture and release of biomolecules by tuning the binding behavior of DNA tetrahedral bioprobes.

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