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Phys Chem Chem Phys. 2016 Aug 3;18(31):21871-80. doi: 10.1039/c6cp03536a.

Influence of PPH dendrimers' surface functions on the activation of human monocytes: a study of their interactions with pure lipid model systems.

Author information

1
Laboratoire IMRCP, UMR 5623 CNRS, Université Toulouse 3, 118 route de Narbonne, F-31062 Toulouse, France. blanzat@chimie.ups-tlse.fr.
2
Laboratoire de Chimie de Coordination du CNRS, UPR 8241, 205 route de Narbonne, BP 44099, F-31077 Toulouse cedex 04, France. turrin@lcc-toulouse.fr and Université de Toulouse, UPS, INPT, LCC, F-31077 Toulouse, France and Centre de Physiopathologie de Toulouse-Purpan, INSERM 1043, CNRS 5282, Université de Toulouse, F-31300 Toulouse, France. remy.poupot@inserm.fr.
3
Laboratoire IMRCP, UMR 5623 CNRS, Université Toulouse 3, 118 route de Narbonne, F-31062 Toulouse, France. blanzat@chimie.ups-tlse.fr and Laboratoire de Chimie de Coordination du CNRS, UPR 8241, 205 route de Narbonne, BP 44099, F-31077 Toulouse cedex 04, France. turrin@lcc-toulouse.fr and Université de Toulouse, UPS, INPT, LCC, F-31077 Toulouse, France.
4
Centre de Physiopathologie de Toulouse-Purpan, INSERM 1043, CNRS 5282, Université de Toulouse, F-31300 Toulouse, France. remy.poupot@inserm.fr.
5
Laboratoire de Chimie de Coordination du CNRS, UPR 8241, 205 route de Narbonne, BP 44099, F-31077 Toulouse cedex 04, France. turrin@lcc-toulouse.fr and Université de Toulouse, UPS, INPT, LCC, F-31077 Toulouse, France.

Abstract

The influence of surface functions on the interactions between Poly(PhosphorHydrazone) PPH dendrimers and human monocytes is discussed on the basis of complementary biological and physicochemical studies on membrane models (monolayers and multi-lamellar vesicles). The studies were performed on both an active and non-toxic phosphonic acid capped dendrimer and a non-active but toxic carboxylic acid capped one. On the one hand, comparative studies of the behaviour of DPPC monolayers in the presence or absence of PPH dendrimers in the subphase showed differences in the phase transitions, highlighting interactions between both dendrimers and phospholipid monolayers, with a larger incidence for the carboxylic acid capped dendrimer (negative control), validating its cellular toxicity. On the other hand, comparative biological studies (activation of human monocytes and binding of fluorescent dendrimers on human monocytes) show the pre-eminence of phosphonic acid capped dendrimers towards specific binding and subsequent activation of human monocytes.

PMID:
27435630
DOI:
10.1039/c6cp03536a
[Indexed for MEDLINE]

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