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J Chromatogr A. 2016 Aug 19;1460:51-60. doi: 10.1016/j.chroma.2016.06.085. Epub 2016 Jul 1.

High throughput peptide mapping method for analysis of site specific monoclonal antibody oxidation.

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Bioprocess Development, Merck Research Laboratories, Merck & Co., Inc., Kenilworth, NJ, 07033, USA.
Bioprocess Development, Merck Research Laboratories, Merck & Co., Inc., Kenilworth, NJ, 07033, USA. Electronic address:


Oxidation of therapeutic monoclonal antibodies (mAbs) often occurs on surface exposed methionine and tryptophan residues during their production in cell culture, purification, and storage, and can potentially impact the binding to their targets. Characterization of site specific oxidation is critical for antibody quality control. Antibody oxidation is commonly determined by peptide mapping/LC-MS methods, which normally require a long (up to 24h) digestion step. The prolonged sample preparation procedure could result in oxidation artifacts of susceptible methionine and tryptophan residues. In this paper, we developed a rapid and simple UV based peptide mapping method that incorporates an 8-min trypsin in-solution digestion protocol for analysis of oxidation. This method is able to determine oxidation levels at specific residues of a mAb based on the peptide UV traces within‚ÄČ<1h, from either TBHP treated or UV light stressed samples. This is the simplest and fastest method reported thus far for site specific oxidation analysis, and can be applied for routine or high throughput analysis of mAb oxidation during various stability and degradation studies. By using the UV trace, the method allows more accurate measurement than mass spectrometry and can be potentially implemented as a release assay. It has been successfully used to monitor antibody oxidation in real time stability studies.


High throughput; Methionine; Monoclonal antibody; Oxidation; Tryptophan

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