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Diabetes. 2016 Oct;65(10):2990-3001. doi: 10.2337/db16-0039. Epub 2016 Jul 18.

Insulin Resistance, Microbiota, and Fat Distribution Changes by a New Model of Vertical Sleeve Gastrectomy in Obese Rats.

Author information

1
Surgical-Medical Department for Digestive Diseases, Policlinico Umberto I, University of Rome Sapienza, Rome, Italy.
2
Computational Biology, Research and Innovation Centre-Fondazione Edmund Mach, San Michele all'Adige, Trento, Italy.
3
Department of Food Quality and Nutrition, Research and Innovation Centre-Fondazione Edmund Mach, San Michele all'Adige, Trento, Italy.
4
Institute of Cardiology, Catholic University, Rome, Italy.
5
Department of Medicine III, Universitätsklinikum Carl Gustav Carus an der Technischen Universität Dresden, Dresden, Germany Diabetes and Nutritional Sciences, King's College London, London, U.K.
6
Research Unit for Multifactorial Diseases, Scientific Directorate, Bambino Gesù Children Hospital, Rome, Italy.
7
Diabetes and Nutritional Sciences, King's College London, London, U.K. Department of Internal Medicine, Catholic University, Rome, Italy geltrude.mingrone@unicatt.it geltrude.mingrone@kcl.ac.uk.

Abstract

Metabolic surgery improves insulin resistance and type 2 diabetes possibly because of weight loss. We performed a novel sleeve gastrectomy in rats that resects ∼80% of the glandular portion, leaving the forestomach almost intact (glandular gastrectomy [GG]) and compared subsequent metabolic remodeling with a sham operation. GG did not affect body weight, at least after 10 weeks; improved hepatic and peripheral insulin sensitivity likely through increased Akt, glycogen synthase kinase 3, and AMPK phosphorylation; and reduced ectopic fat deposition and hepatic glycogen overaccumulation. Body adipose tissue was redistributed, with reduction of intraabdominal fat. We found a reduction of circulating ghrelin levels, increased GLP-1 plasma concentration, and remodeling of gut microbiome diversity characterized by a lower relative abundance of Ruminococcus and a higher relative abundance of Lactobacillus and Collinsella These data suggest that at least in rat, the glandular stomach plays a central role in the improvement of insulin resistance, even if obesity persists. GG provides a new model of the metabolically healthy obese phenotype.

PMID:
27431457
DOI:
10.2337/db16-0039
[Indexed for MEDLINE]
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