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Nat Methods. 2016 Sep;13(9):755-8. doi: 10.1038/nmeth.3926. Epub 2016 Jul 18.

LOVTRAP: an optogenetic system for photoinduced protein dissociation.

Author information

1
Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.
2
Lyda Hill Department of Bioinformatics, UT Southwestern Medical Center, Dallas, Texas, USA.
3
Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Heidelberg, Germany.
4
Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.
5
Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.
6
Carolina Center for Genome Sciences, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.
7
Lineberger Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.

Abstract

LOVTRAP is an optogenetic approach for reversible light-induced protein dissociation using protein A fragments that bind to the LOV domain only in the dark, with tunable kinetics and a >150-fold change in the dissociation constant (Kd). By reversibly sequestering proteins at mitochondria, we precisely modulated the proteins' access to the cell edge, demonstrating a naturally occurring 3-mHz cell-edge oscillation driven by interactions of Vav2, Rac1, and PI3K proteins.

PMID:
27427858
PMCID:
PMC5137947
DOI:
10.1038/nmeth.3926
[Indexed for MEDLINE]
Free PMC Article

Conflict of interest statement

COMPETING FINACIAL INTERESTS STATEMENT The authors declare no competing financial interests.

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