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Phytother Res. 2016 Oct;30(10):1696-1707. doi: 10.1002/ptr.5678. Epub 2016 Jul 18.

The Water Fraction of Calendula officinalis Hydroethanol Extract Stimulates In Vitro and In Vivo Proliferation of Dermal Fibroblasts in Wound Healing.

Author information

1
Department of Life Science and Biotechnology, Jadavpur University, 188, Raja S.C. Mullick Road, Kolkata, 700 032, West Bengal, India.
2
Department of Chemistry (Inorganic Section), Jadavpur University, 188, Raja S.C. Mullick Road, Kolkata, 700 032, West Bengal, India.
3
Division of Molecular Medicine, Bose Institute, P 1/12, CIT Scheme VIIM, Kolkata, 700 054, India.
4
Department of Life Science and Biotechnology, Jadavpur University, 188, Raja S.C. Mullick Road, Kolkata, 700 032, West Bengal, India. pkarmakar_28@yahoo.co.in.

Abstract

The active fraction and/or compounds of Calendula officinalis responsible for wound healing are not known yet. In this work we studied the molecular target of C. officinalis hydroethanol extract (CEE) and its active fraction (water fraction of hydroethanol extract, WCEE) on primary human dermal fibroblasts (HDF). In vivo, CEE or WCEE were topically applied on excisional wounds of BALB/c mice and the rate of wound contraction and immunohistological studies were carried out. We found that CEE and only its WCEE significantly stimulated the proliferation as well as the migration of HDF cells. Also they up-regulate the expression of connective tissue growth factor (CTGF) and α-smooth muscle actin (α-SMA) in vitro. In vivo, CEE or WCEE treated mice groups showed faster wound healing and increased expression of CTGF and α-SMA compared to placebo control group. The increased expression of both the proteins during granulation phase of wound repair demonstrated the potential role of C. officinalis in wound healing. In addition, HPLC-ESI MS analysis of the active water fraction revealed the presence of two major compounds, rutin and quercetin-3-O-glucoside. Thus, our results showed that C. officinalis potentiated wound healing by stimulating the expression of CTGF and α-SMA and further we identified active compounds.

KEYWORDS:

CTGF; Calendula officinalis hydroethanol extract (CEE); water fraction of Calendula officinalis hydroethanol extract (WCEE); wound healing; α-SMA

PMID:
27426257
DOI:
10.1002/ptr.5678
[Indexed for MEDLINE]

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