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Gene. 2016 Oct 10;591(1):255-261. doi: 10.1016/j.gene.2016.07.031. Epub 2016 Jul 15.

Studies on the biological functions of CPS1 in AFB1 induced hepatocarcinogenesis.

Author information

1
Key Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province, Key Laboratory of Biopesticide and Chemical Biology of the Education Ministry, School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Institute of Edible Fungi, National and Local Joint Engineering Research Center for Breeding & Cultivation of Featured Edible Fungi, Fujian Academy of Agricultural Sciences, Fuzhou 350014, China.
2
Key Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province, Key Laboratory of Biopesticide and Chemical Biology of the Education Ministry, School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
3
Key Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province, Key Laboratory of Biopesticide and Chemical Biology of the Education Ministry, School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China. Electronic address: wshyyl@sina.com.

Abstract

Carbamyl phosphate synthetase 1 (CPS1) was down-regulated in hepatocellular carcinoma (HCC), as treated by aflatoxin B1 (AFB1), a potent hepatocarcinogenesis mycotoxin. In this study, we firstly confirmed that AFB1 down-regulated the expression of CPS1 in a dose-dependent manner. At the meantime, both siRNA knock down of CPS1 and AFB1 treatment inhibited cell proliferation, and induced cell apoptosis. To further analysis the function of CPS1, the interacting proteins of CPS1 were searched by Co-IP, and three interacting proteins including type II cytoskeletal 1 (KRT1), albumin (ALB), and ubiquitin C (UBC) were found. Both KRT1 and ALB were new interacting proteins for CPS1. Our further study showed that CPS1 was regulating interacted and colocalized with KRT1 and ALB, and the intensity correlation was changed by AFB1. KRT1, ALB and CPS1 were all reported to play an important role in differentiation and tissue specialization. These results may offer an increasing understand that CPS1 might have a function in differentiation.

KEYWORDS:

Aflatoxin B(1); Carbamyl phosphate synthetase I; Hepatocarcinogenesis; Interacting protein

PMID:
27425868
DOI:
10.1016/j.gene.2016.07.031
[Indexed for MEDLINE]

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