Bioluminogenic Imaging of AminopeptidaseN In Vitro and In Vivo

Wenxiao Wu; Laizhong Chen; Jing Li; Lupei Du; Minyong Li

doi:10.1007/978-1-4939-3813-1_7

Bioluminogenic Imaging of AminopeptidaseN In Vitro and In Vivo

Methods Mol Biol. 2016:1461:91-9. doi: 10.1007/978-1-4939-3813-1_7.

Authors

Wenxiao Wu¹, Laizhong Chen¹, Jing Li¹, Lupei Du¹, Minyong Li²

Affiliations

¹ Department of Medicinal Chemistry, Key Laboratory of Chemical Biology of Natural Products (MOE), School of Pharmacy, Shandong University, Jinan, Shandong, 250012, China.
² Department of Medicinal Chemistry, Key Laboratory of Chemical Biology of Natural Products (MOE), School of Pharmacy, Shandong University, Jinan, Shandong, 250012, China. mli@sdu.edu.cn.

PMID: 27424897
DOI: 10.1007/978-1-4939-3813-1_7

Abstract

Bioluminescence is a process that converts biochemical energy to visible light. Bioluminescence-based imaging technology has been widely applicable in the imaging of process envisioned in life sciences. As one of the most popular bioluminescence system, the firefly luciferin-luciferase system is exceptionally suitable for deep tissue imaging in living animals owing to its long wavelength emission light. Herein, we report the experimental detail of bioluminogenic imaging of aminopeptidase N activity both in vitro and in vivo.

Keywords: Aminopeptidase N; Bioluminescence; Caged luciferin; Living animal imaging.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
CD13 Antigens / metabolism*
Cell Line
In Vitro Techniques
Luciferases, Firefly / metabolism
Luminescent Agents
Luminescent Measurements / methods*
Mice
Molecular Imaging / methods*

Substances

Luminescent Agents
Luciferases, Firefly
CD13 Antigens