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Trends Genet. 2016 Sep;32(9):526-529. doi: 10.1016/j.tig.2016.06.004. Epub 2016 Jul 13.

CRISPR Screens to Discover Functional Noncoding Elements.

Author information

1
Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, MA 02142, USA; McGovern Institute for Brain Research, Department of Brain and Cognitive Sciences, Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
2
New York Genome Center, New York, NY 10013, USA; Center for Genomics and Systems Biology, Department of Biology, New York University, NY 10003, USA. Electronic address: nsanjana@nygenome.org.

Abstract

A major challenge in genomics is to identify functional elements in the noncoding genome. Recently, pooled clustered regularly interspersed palindromic repeat (CRISPR) mutagenesis screens of noncoding regions have emerged as a novel method for finding elements that impact gene expression and phenotype/disease-relevant biological processes. Here we review and compare different approaches for high-throughput dissection of noncoding elements.

KEYWORDS:

CRISPR; Cas9; enhancer; functional genomics; gene regulation; noncoding

PMID:
27423542
PMCID:
PMC4992445
DOI:
10.1016/j.tig.2016.06.004
[Indexed for MEDLINE]
Free PMC Article

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