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J Exp Bot. 2016 Sep;67(17):5247-58. doi: 10.1093/jxb/erw283. Epub 2016 Jul 15.

A soybean quantitative trait locus that promotes flowering under long days is identified as FT5a, a FLOWERING LOCUS T ortholog.

Author information

1
Research Faculty of Agriculture, Hokkaido University, Sapporo, Hokkaido 060-8589, Japan.
2
The Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin 150081, China.
3
Hokkaido Research Organization Tokachi Agricultural Experiment Station, Memuro, Hokkaido 082-0081, Japan.
4
National Institute of Agrobiological Sciences, Kannondai, Ibaraki 305-8602, Japan.
5
State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 1001014, China.
6
The Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin 150081, China kongfj@iga.ac.cn jabe@res.agr.hokucai.ac.jp.
7
Research Faculty of Agriculture, Hokkaido University, Sapporo, Hokkaido 060-8589, Japan kongfj@iga.ac.cn jabe@res.agr.hokucai.ac.jp.

Abstract

FLOWERING LOCUS T (FT) is an important floral integrator whose functions are conserved across plant species. In soybean, two orthologs, FT2a and FT5a, play a major role in initiating flowering. Their expression in response to different photoperiods is controlled by allelic combinations at the maturity loci E1 to E4, generating variation in flowering time among cultivars. We determined the molecular basis of a quantitative trait locus (QTL) for flowering time in linkage group J (Chromosome 16). Fine-mapping delimited the QTL to a genomic region of 107kb that harbors FT5a We detected 15 DNA polymorphisms between parents with the early-flowering (ef) and late-flowering (lf) alleles in the promoter region, an intron, and the 3' untranslated region of FT5a, although the FT5a coding regions were identical. Transcript abundance of FT5a was higher in near-isogenic lines for ef than in those for lf, suggesting that different transcriptional activities or mRNA stability caused the flowering time difference. Single-nucleotide polymorphism (SNP) calling from re-sequencing data for 439 cultivated and wild soybean accessions indicated that ef is a rare haplotype that is distinct from common haplotypes including lf The ef allele at FT5a may play an adaptive role at latitudes where early flowering is desirable.

KEYWORDS:

FLOWERING LOCUS T; SNP calling; flowering time; near-isogenic line; photoperiod sensitivity; quantitative trait locus; soybean.

PMID:
27422993
PMCID:
PMC5014162
DOI:
10.1093/jxb/erw283
[Indexed for MEDLINE]
Free PMC Article

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