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Neurosci Lett. 2016 Aug 26;629:171-179. doi: 10.1016/j.neulet.2016.07.014. Epub 2016 Jul 12.

Brain-wide map of projections from mice ventral subiculum.

Author information

1
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan 650201, China; University of Chinese Academy of Sciences, Beijing 100039, China.
2
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan 650201, China.
3
State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan 430071, China.
4
State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan 430071, China; Wuhan National Laboratory for Optoelectronics, Wuhan 430074, China. Electronic address: fuqiang.xu@wipm.ac.cn.
5
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan 650201, China. Electronic address: luohuairong@mail.kib.ac.cn.

Abstract

The hippocampal formation plays a critical role in episodic memory formation and spatial navigation. Within the hippocampus, the subiculum is considered to be a hub connecting the hippocampal formation to the remainder of the brain. There are functional differences between the dorsal and ventral part of subiculum, while the ventral subiculum (vSub) plays a role in anxiety, stress and emotion. In the present study, we examined the projection of the ventral subiculum to the whole brain in mice by using a modified herpes simplex virus 1 strain H129 with an inserted fluorescent protein gene. In our experiments, the modified H129 transits the primary-order, second-order, and third-order neuronal projections at 36-44, 52-60 and 68-76h after inoculation in mice, respectively. Our data revealed that vSub directly projects to the medial entorhinal cortex, amygdalohippocampal area, anterodorsal thalamic nucleus, medial hypothalamus, supramammillary nucleus, medial septal nucleus and adjacent diagonal band, the connections between median raphe nucleus and interpeduncular nucleus in brain stem, while ventral prefrontal cortex, laterodorsal tegmental nucleus and locus coeruleus receives second-order projections from vSub. Our data would help further understanding the functional connections of vSub with other brain regions.

KEYWORDS:

Brain map; Herpes simplex virus; Hippocampal formation; Neural circuits; Ventral subiculum

PMID:
27422730
DOI:
10.1016/j.neulet.2016.07.014
[Indexed for MEDLINE]

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