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Biochim Biophys Acta. 2016 Nov;1861(11):1671-1680. doi: 10.1016/j.bbalip.2016.07.003. Epub 2016 Jul 12.

Genes and miRNA expression signatures in peripheral blood mononuclear cells in healthy subjects and patients with metabolic syndrome after acute intake of extra virgin olive oil.

Author information

1
Department of Interdisciplinary Medicine, Clinica Medica "C. Frugoni", Aldo Moro University of Bari, Italy; National Cancer Research Center, IRCCS Oncologico Giovanni Paolo II, Bari, Italy; Department of Biomedical Sciences and Human Oncology, Clinica Medica "A. Murri", Aldo Moro University of Bari, Italy.
2
Laboratory of Lipid Metabolism and Cancer, Consorzio Mario Negri Sud, Santa Maria Imbaro, Chieti, Italy.
3
Department of Interdisciplinary Medicine, Clinica Medica "C. Frugoni", Aldo Moro University of Bari, Italy.
4
National Cancer Research Center, IRCCS Oncologico Giovanni Paolo II, Bari, Italy.
5
Department of Biomedical Sciences and Human Oncology, Clinica Medica "A. Murri", Aldo Moro University of Bari, Italy.
6
Department of Interdisciplinary Medicine, Clinica Medica "C. Frugoni", Aldo Moro University of Bari, Italy. Electronic address: antonio.moschetta@uniba.it.

Abstract

Extra virgin olive oil (EVOO) consumption has been associated with reduced cardiovascular risk but molecular mechanisms underlying its beneficial effects are not fully understood. Here we aimed to identify genes and miRNAs expression changes mediated by acute high- and low-polyphenols EVOO intake. Pre- and post-challenge gene and miRNAs expression analysis was performed on the peripheral blood mononuclear cells (PBMCs) of 12 healthy subjects and 12 patients with metabolic syndrome (MS) by using microarray and RT-qPCR. In healthy subjects, acute intake of EVOO rich in polyphenols was able to ameliorate glycaemia and insulin sensitivity, and to modulate the transcription of genes and miRNAs involved in metabolism, inflammation and cancer, switching PBMCs to a less deleterious inflammatory phenotype; weaker effects were observed in patients with MS as well as in healthy subjects following low-polyphenol EVOO challenge. Concluding, our study shows that acute high-polyphenols EVOO intake is able to modify the transcriptome of PBMCs through the modulation of different pathways associated with the pathophysiology of cardio-metabolic disease and cancer. These beneficial effects are maximized in healthy subjects, and by the use of EVOO cultivars rich in polyphenols. Nutrigenomic changes induced by EVOO thus legitimate the well-known beneficial effects of EVOO in promoting human health and, potentially, preventing the onset of cardiovascular disease and cancer.

KEYWORDS:

Extra virgin olive oil; Gene expression; PBMCs; miRNAs

PMID:
27422371
DOI:
10.1016/j.bbalip.2016.07.003
[Indexed for MEDLINE]

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