Format

Send to

Choose Destination
Am J Transplant. 2017 Jan;17(1):239-245. doi: 10.1111/ajt.13964. Epub 2016 Aug 18.

Adipose Gene Expression Profile Changes With Lung Allograft Reperfusion.

Author information

1
Pulmonary, Allergy, and Critical Care Division, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA.
2
Division of Pulmonary, Allergy, and Critical Care Medicine, Columbia University College of Physicians and Surgeons, New York, New York.
3
Department of Surgery, Columbia University College of Physicians and Surgeons, New York, New York.
4
Division of Cardiovascular Surgery, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA.
5
Division of Rheumatology, Columbia University College of Physicians and Surgeons, New York, New York.
6
Department of Medicine, Naomi Berrie Diabetes Center, Columbia University, New York, New York.
7
Division of Cardiology, Columbia University College of Physicians and Surgeons, New York, New York.
#
Contributed equally

Abstract

Obesity is a risk factor for primary graft dysfunction (PGD), a form of lung injury resulting from ischemia-reperfusion after lung transplantation, but the impact of ischemia-reperfusion on adipose tissue is unknown. We evaluated differential gene expression in thoracic visceral adipose tissue (VAT) before and after lung reperfusion. Total RNA was isolated from thoracic VAT sampled from six subjects enrolled in the Lung Transplant Body Composition study before and after allograft reperfusion and quantified using the Human Gene 2.0 ST array. Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed enrichment for genes involved in complement and coagulation cascades and Jak-STAT signaling pathways. Overall, 72 genes were upregulated and 56 genes were downregulated in the postreperfusion time compared with baseline. Long pentraxin-3, a gene and plasma protein previously associated with PGD, was the most upregulated gene (19.5-fold increase, p = 0.04). Fibronectin leucine-rich transmembrane protein-3, a gene associated with cell adhesion and receptor signaling, was the most downregulated gene (4.3-fold decrease, p = 0.04). Ischemia-reperfusion has a demonstrable impact on gene expression in visceral adipose tissue in our pilot study of nonobese, non-PGD lung transplant recipients. Future evaluation will focus on differential adipose tissue gene expression and the development of PGD after transplant.

KEYWORDS:

genomics; ischemia reperfusion injury (IRI); lung (allograft) function/dysfunction; lung transplantation/pulmonology; translational research/science

PMID:
27421969
PMCID:
PMC5195853
DOI:
10.1111/ajt.13964
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Wiley Icon for PubMed Central
Loading ...
Support Center