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Anal Biochem. 1989 Feb 15;177(1):110-4.

Estimation of circular DNA size using gamma-irradiation and pulsed-field gel electrophoresis.

Author information

1
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115.

Abstract

A method is described for estimating the size of large circular DNAs found within complex chromosomal DNA preparations. DNAs are treated with low levels of gamma-irradiation, sufficient to introduce a single double-stranded break per circle, and the resulting linear DNA is sized by pulsed-field electrophoresis and blot hybridization. The method is fast, reproducible, and very conveniently applied to the agarose-enclosed chromosomal DNA preparations commonly used in pulsed field electrophoresis.

PMID:
2742141
DOI:
10.1016/0003-2697(89)90023-7
[Indexed for MEDLINE]

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