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Int J Urol. 2016 Oct;23(10):881-887. doi: 10.1111/iju.13158. Epub 2016 Jul 14.

Effects of silodosin on bladder activity in rats with frequent urination induced by pelvic venous congestion.

Author information

1
Southern Knights' Laboratory, Okinawa, Japan. sugaya@sklabo.com.
2
Southern Knights' Laboratory, Okinawa, Japan.
3
Faculty of Medicine, The Institute for Animal Experiments, University of the Ryukyus, Okinawa, Japan.
4
Department of Biochemistry, Graduate School of Medicine, University of the Ryukyus, Okinawa, Japan.

Abstract

OBJECTIVE:

To examine the effects of silodosin on bladder activity using female rats with frequent urination induced by pelvic venous congestion.

METHODS:

A total of 24 female rats were divided into three groups: sham, pelvic venous congestion and pelvic venous congestion/silodosin group. Rats in the pelvic venous congestion and pelvic venous congestion/silodosin groups were anesthetized with isoflurane, after which the bilateral common iliac veins and uterine veins were ligated. In the pelvic venous congestion/silodosin group, silodosin (0.3 mg/kg/day) was given using an osmotic pump implanted into the subcutaneous space of the back. After 5-6 weeks, analysis of voiding behavior, measurements of urinary 8-hydroxydeoxyguanosine and nitric oxide metabolites, continuous cystometry under urethane anesthesia, and Evans blue dye extravasation test of the bladder were carried out.

RESULTS:

In comparison with sham rats, pelvic venous congestion rats showed an increase in urination frequency with a concomitant increase in urine volume, a shorter interval between bladder contractions on continuous cystometry, an increase in urinary 8-hydroxydeoxyguanosine, a decrease in urinary nitric oxide metabolites and an increase in vesical vascular permeability. In comparison with pelvic venous congestion rats, pelvic venous congestion/silodosin rats showed a decrease in urination frequency with a concomitant decrease in urine volume, a lower maximum bladder contraction pressure, a longer interval between bladder contractions, an increase in urinary nitric oxide metabolites and a decrease in vascular permeability.

CONCLUSION:

Silodosin might improve both bladder dysfunction caused by pelvic venous congestion, and the pelvic venous congestion itself.

KEYWORDS:

nitric oxide; pelvic venous congestion; silodosin; urinary frequency; vascular permeability

PMID:
27418315
DOI:
10.1111/iju.13158
[Indexed for MEDLINE]
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