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J Biomed Mater Res B Appl Biomater. 2017 Oct;105(7):2136-2144. doi: 10.1002/jbm.b.33751. Epub 2016 Jul 15.

Novel fabrication method of the peritoneal dialysis filter using silk fibroin with urease fixation system.

Author information

1
Nano-Bio Regenerative Medical Institute, College of Medicine, Hallym University, Chuncheon, 200-702, Republic of Korea.
2
Department of Nephrology, Chuncheon Sacred Heart Hospital, Hallym University College of Medicine, Chuncheon, 200-704, Republic of Korea.
3
Department of Nephrology, Yonsei University Wonju College of Medicine, Wonju, 220-701, Republic of Korea.
4
Department of Materials Science and Engineering College of Information and Electronic Engineering, Hallym University, Chuncheon, 200-702, Republic of Korea.
5
Department of Otorhinolaryngology-Head and Neck Surgery, Chuncheon Sacred Heart Hospital, Hallym University College of Medicine, Chuncheon, 200-704, Republic of Korea.

Abstract

During the last decade, there has been a great advance in the kidney dialysis system by wearable artificial kidney (WAK) system for end-stage renal disease patients. Uremic solute removal and water regeneration system are the most prerequisite for WAK to work properly. In this study, we designed a filtering membrane system by using immobilized urease silk fibroin filter and evaluated its comparative effectiveness with a PVDF filtering system in peritoneal dialysate regeneration system by urea removal efficacy. We evaluated this membrane's characteristic and performances by conducting SEM-EDX analyze, water-binding abilities and porosity test, removal abilities of urea, cytotoxicity assay and enzyme activity assay. Under the condition for optimization of urease, the percentage removal of urea was about 40% and 60% in 50 mg/dL urea solution by urease immobilized PVDF and silk fibroin scaffolds, respectively. The batch experimental result showed that immobilized filter removed more than 50% of urea in 50 mg/dL urea solution. In addition silk fibroin with urease filter removed 90 percent of urea in the peritoneal dialysate after 24 h filtration. We suggest that silk fibroin with urease fixation filter can be used more effectively for peritoneal dialysate regeneration system, which have hydrophilic property and prolonged enzyme activity.

KEYWORDS:

bio-filter; peritoneal dialysis; silk fibroin; urea filter; wearable artificial kidney

PMID:
27417290
DOI:
10.1002/jbm.b.33751
[Indexed for MEDLINE]

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