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J Pharm Biomed Anal. 2016 Sep 10;129:105-116. doi: 10.1016/j.jpba.2016.06.038. Epub 2016 Jun 23.

An efficient approach to identify different chemical markers between fibrous root and rhizome of Anemarrhena asphodeloides by ultra high-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry with multivariate statistical analysis.

Author information

1
Beijing Institute of Radiation Medicine, Beijing 100850, PR China; Tianjin University of Traditional Chinese Medicine, Tianjin 300193, PR China.
2
Beijing Institute of Radiation Medicine, Beijing 100850, PR China.
3
Ovation Health Science and Technology Co. Ltd., ENN Group, Langfang 065001, PR China.
4
Beijing Institute of Radiation Medicine, Beijing 100850, PR China. Electronic address: mabaiping@sina.com.

Abstract

An ultra high-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry approach coupled with multivariate statistical analysis was established and applied to rapidly distinguish the chemical differences between fibrous root and rhizome of Anemarrhena asphodeloides. The datasets of tR-m/z pairs, ion intensity and sample code were processed by principal component analysis and orthogonal partial least squares discriminant analysis. Chemical markers could be identified based on their exact mass data, fragmentation characteristics, and retention times. And the new compounds among chemical markers could be isolated rapidly guided by the ultra high-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry and their definitive structures would be further elucidated by NMR spectra. Using this approach, twenty-four markers were identified on line including nine new saponins and five new steroidal saponins of them were obtained in pure form. The study validated this proposed approach as a suitable method for identification of the chemical differences between various medicinal parts in order to expand medicinal parts and increase the utilization rate of resources.

KEYWORDS:

Anemarrhena asphodeloides; Multivariate statistical analysis; Steroidal saponins; Structure elucidation; UHPLC-Q-TOF MS(E)

PMID:
27416524
DOI:
10.1016/j.jpba.2016.06.038
[Indexed for MEDLINE]

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