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PLoS Biol. 2016 Jul 14;14(7):e1002515. doi: 10.1371/journal.pbio.1002515. eCollection 2016 Jul.

Topological Small-World Organization of the Fibroblastic Reticular Cell Network Determines Lymph Node Functionality.

Author information

1
Institute of Immunobiology, Kantonsspital St. Gallen, St. Gallen, Switzerland.
2
Theodor Kocher Institute, University of Bern, Bern, Switzerland.
3
Novartis Institutes for Biomedical Research, Cambridge, Massachusetts, United States of America.
4
Institute of Numerical Mathematics, Russian Academy of Sciences, Moscow, Russia.
5
Department of Cancer Immunology, Genentech, South San Francisco, California, United States of America.

Abstract

Fibroblastic reticular cells (FRCs) form the cellular scaffold of lymph nodes (LNs) and establish distinct microenvironmental niches to provide key molecules that drive innate and adaptive immune responses and control immune regulatory processes. Here, we have used a graph theory-based systems biology approach to determine topological properties and robustness of the LN FRC network in mice. We found that the FRC network exhibits an imprinted small-world topology that is fully regenerated within 4 wk after complete FRC ablation. Moreover, in silico perturbation analysis and in vivo validation revealed that LNs can tolerate a loss of approximately 50% of their FRCs without substantial impairment of immune cell recruitment, intranodal T cell migration, and dendritic cell-mediated activation of antiviral CD8+ T cells. Overall, our study reveals the high topological robustness of the FRC network and the critical role of the network integrity for the activation of adaptive immune responses.

PMID:
27415420
PMCID:
PMC4945005
DOI:
10.1371/journal.pbio.1002515
[Indexed for MEDLINE]
Free PMC Article

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