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Stem Cell Reports. 2016 Jul 12;7(1):126-37. doi: 10.1016/j.stemcr.2016.06.004.

Immobilized WNT Proteins Act as a Stem Cell Niche for Tissue Engineering.

Author information

1
Centre for Stem Cells and Regenerative Medicine, King's College London, London SE1 9RT, UK.
2
Institute for Science and Technology in Medicine, Guy Hilton Research Centre, Keele University, Stoke-on-Trent ST4 7QB, UK.
3
Centre for Stem Cells and Regenerative Medicine, King's College London, London SE1 9RT, UK; Institute for Science and Technology in Medicine, Guy Hilton Research Centre, Keele University, Stoke-on-Trent ST4 7QB, UK.
4
Centre for Stem Cells and Regenerative Medicine, King's College London, London SE1 9RT, UK. Electronic address: shukry.habib@kcl.ac.uk.

Abstract

The timing, location, and level of WNT signaling are highly regulated during embryonic development and for the maintenance of adult tissues. Consequently the ability to provide a defined and directed source of WNT proteins is crucial to fully understand its role in tissue development and to mimic its activity in vitro. Here we describe a one-step immobilization technique to covalently bind WNT3A proteins as a basal surface with easy storage and long-lasting activity. We show that this platform is able to maintain adult and embryonic stem cells while also being adaptable for 3D systems. Therefore, this platform could be used for recapitulating specific stem cell niches with the goal of improving tissue engineering.

PMID:
27411105
PMCID:
PMC4944585
DOI:
10.1016/j.stemcr.2016.06.004
[Indexed for MEDLINE]
Free PMC Article

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