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Invest Ophthalmol Vis Sci. 2016 Jul 1;57(9):OCT86-95. doi: 10.1167/iovs.15-18891.

Visible-Light Optical Coherence Tomography Angiography for Monitoring Laser-Induced Choroidal Neovascularization in Mice.

Author information

1
Department of Ophthalmology Feinberg School of Medicine, Northwestern University, Chicago, Illinois, United States.
2
Department of Ophthalmology Feinberg School of Medicine, Northwestern University, Chicago, Illinois, United States 2Functional Optical Imaging Laboratory, Department of Biomedical Engineering, Northwestern University, Chicago, Illinois, United States 3Med.
3
Functional Optical Imaging Laboratory, Department of Biomedical Engineering, Northwestern University, Chicago, Illinois, United States.
4
Department of Ophthalmology Feinberg School of Medicine, Northwestern University, Chicago, Illinois, United States 2Functional Optical Imaging Laboratory, Department of Biomedical Engineering, Northwestern University, Chicago, Illinois, United States.

Abstract

PURPOSE:

This study sought to determine the earliest time-point at which evidence of choroidal neovascularization (CNV) could be detected with visible-light optical coherence tomography angiography (vis-OCTA) in a mouse model of laser-induced CNV.

METHODS:

Visible light-OCTA was used to study laser-induced CNV at different time-points after laser injury to monitor CNV development and measure CNV lesion size. Measurements obtained from vis-OCTA angiograms were compared with histopathologic measurements from isolectin-stained choroidal flatmounts.

RESULTS:

Choroidal neovascularization area measurements between the vis-OCTA system and isolectin-stained choroidal flatmounts were significantly different in area for days 2 to 4 postlaser injury, and were not significantly different in area for days 5, 7, and 14. Choroidal neovascularization area measurements taken from the stained flatmounts were larger than their vis-OCTA counterparts for all time-points. Both modalities showed a similar trend of CNV size increasing from the day of laser injury until a peak of day 7 postlaser injury and subsequently decreasing by day 14.

CONCLUSIONS:

The earliest vis-OCTA can detect the presence of aberrant vessels in a mouse laser-induced CNV model is 5 days after laser injury. Visible light-OCTA was able to visualize the maximum of the CNV network 7 days postlaser injury, in accordance with choroidal flatmount immunostaining. Visible light-OCTA is a reliable tool in both detecting the presence of CNV development, as well as accurately determining the size of the lesion in a mouse laser-induced CNV model.

PMID:
27409510
PMCID:
PMC4968775
DOI:
10.1167/iovs.15-18891
[Indexed for MEDLINE]
Free PMC Article

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