Format

Send to

Choose Destination
Nucleic Acids Res. 2016 Nov 2;44(19):9190-9205. Epub 2016 Jul 8.

A subset of replication-dependent histone mRNAs are expressed as polyadenylated RNAs in terminally differentiated tissues.

Author information

1
Department of Biology, University of North Carolina, Chapel Hill, NC 27599, USA.
2
Department of Computer Science, University of North Carolina, Chapel Hill, NC 27599.
3
Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC 27599, USA.
4
Department of Genetics, Dartmouth Geisel School of Medicine, Hanover, NH 03755, USA.
5
Integrative Program for Biological and Genome Sciences, University of North Carolina, Chapel Hill, NC 27599, USA.
6
Department of Biology, University of North Carolina, Chapel Hill, NC 27599, USA marzluff@med.unc.edu.

Abstract

Histone proteins are synthesized in large amounts during S-phase to package the newly replicated DNA, and are among the most stable proteins in the cell. The replication-dependent (RD)-histone mRNAs expressed during S-phase end in a conserved stem-loop rather than a polyA tail. In addition, there are replication-independent (RI)-histone genes that encode histone variants as polyadenylated mRNAs. Most variants have specific functions in chromatin, but H3.3 also serves as a replacement histone for damaged histones in long-lived terminally differentiated cells. There are no reported replacement histone genes for histones H2A, H2B or H4. We report that a subset of RD-histone genes are expressed in terminally differentiated tissues as polyadenylated mRNAs, likely serving as replacement histone genes in long-lived non-dividing cells. Expression of two genes, HIST2H2AA3 and HIST1H2BC, is conserved in mammals. They are expressed as polyadenylated mRNAs in fibroblasts differentiated in vitro, but not in serum starved fibroblasts, suggesting that their expression is part of the terminal differentiation program. There are two histone H4 genes and an H3 gene that encode mRNAs that are polyadenylated and expressed at 5- to 10-fold lower levels than the mRNAs from H2A and H2B genes, which may be replacement genes for the H3.1 and H4 proteins.

PMID:
27402160
PMCID:
PMC5100578
DOI:
10.1093/nar/gkw620
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Silverchair Information Systems Icon for PubMed Central
Loading ...
Support Center