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Genes Dev. 2016 Jul 1;30(13):1503-8. doi: 10.1101/gad.281188.116.

Mitosis-associated repression in development.

Author information

1
Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey 08544, USA; Department of Molecular and Cell Biology, Division of Genetics, Genomics, and Development, University of California at Berkeley, Berkeley, California 94720, USA;
2
Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey 08544, USA;
3
Department of Physics, Princeton University, Princeton, New Jersey 08544, USA;
4
Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey 08544, USA; Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA.

Abstract

Transcriptional repression is a pervasive feature of animal development. Here, we employ live-imaging methods to visualize the Snail repressor, which establishes the boundary between the presumptive mesoderm and neurogenic ectoderm of early Drosophila embryos. Snail target enhancers were attached to an MS2 reporter gene, permitting detection of nascent transcripts in living embryos. The transgenes exhibit initially broad patterns of transcription but are refined by repression in the mesoderm following mitosis. These observations reveal a correlation between mitotic silencing and Snail repression. We propose that mitosis and other inherent discontinuities in transcription boost the activities of sequence-specific repressors, such as Snail.

KEYWORDS:

Drosophila embryo; live imaging; mitosis; repression; transcription

PMID:
27401553
PMCID:
PMC4949323
DOI:
10.1101/gad.281188.116
[Indexed for MEDLINE]
Free PMC Article

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