Format

Send to

Choose Destination
Gen Comp Endocrinol. 2016 Sep 15;236:70-82. doi: 10.1016/j.ygcen.2016.07.008. Epub 2016 Jul 9.

Gonadotropin-releasing hormone and adipokinetic hormone/corazonin-related peptide in the female prawn.

Author information

1
Department of Anatomy, Faculty of Science, Mahidol University, Bangkok 10400, Thailand; Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, Maroochydore, Queensland 4558, Australia.
2
Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, Maroochydore, Queensland 4558, Australia.
3
Department of Anatomy, Faculty of Science, Mahidol University, Bangkok 10400, Thailand; School of Medicine, Walailak University, Nakhon Si Thammarat 80161, Thailand.
4
Department of Anatomy, Phramongkutklao College of Medicine, Bangkok 10400, Thailand.
5
Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, Maroochydore, Queensland 4558, Australia. Electronic address: scummins@usc.edu.au.
6
Department of Anatomy, Faculty of Science, Mahidol University, Bangkok 10400, Thailand; Faculty of Allied Health Sciences, Burapha University, Chonburi 20131, Thailand. Electronic address: prasert.sob@mahidol.ac.th.

Abstract

Crustacean neuropeptides (NPs) play important roles in the regulation of most physiological activities, including growth, molting and reproduction. In this study, we have performed an in silico analysis of female prawn (Macrobrachium rosenbergii) neural transcriptomes to identify NPs not previously identified. We predict that approximately 1309 proteins are destined for the secretory pathway, many of which are likely post-translationally processed to generate active peptides. Within this neural secretome, we identified a gene transcript that encoded a precursor protein with striking similarity to a gonadotropin-releasing hormone (GnRH). We additionally identified another GnRH NP superfamily member, the adipokinetic hormone/corazonin-related peptide (ACP). M. rosenbergii GnRH and ACP were widespread throughout the nervous tissues, implicating them as potential neuromodulators. Furthermore, GnRH was found in non-neural tissues, including the stomach, gut, heart, testis and ovary, in the latter most prominently within secondary oocytes. The GnRH/corazonin receptor-like gene is specific to the ovary, whereas the receptor-like gene expression is more widespread. Administration of GnRH had no effect on ovarian development and maturation, nor any effect on total hemolymph lipid levels, while ACP administration decreased oocyte proliferation (at high dose) and stimulated a significant increase in total hemolymph lipids. In conclusion, our targeted analysis of the M. rosenbergii neural secretome has revealed the decapod GnRH and ACP genes. We propose that ACP in crustaceans plays a role in the lipid metabolism and the inhibition of oocyte proliferation, while the role of the GnRH remains to be clearly defined, possibly through experiments involving gene silencing.

KEYWORDS:

Adipokinetic hormone/corazonin-related peptide; Crustaceans; Gonadotropin-releasing hormone; Macrobrachium rosenbergii; Neuropeptide

PMID:
27401259
DOI:
10.1016/j.ygcen.2016.07.008
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center