Efficacy of Soybean's Event DAS-81419-2 Expressing Cry1F and Cry1Ac to Manage Key Tropical Lepidopteran Pests Under Field Conditions in Brazil

J Econ Entomol. 2016 Aug;109(4):1922-8. doi: 10.1093/jee/tow153. Epub 2016 Jul 8.

Abstract

Bacillus thuringiensis (Bt) event DAS-81419-2 (Conkesta technology) in soybean, Glycine max (L.) Merrill, expresses Cry1F and Cry1Ac proteins to provide protection from feeding by several lepidopteran pests. A total of 27 field experiments across nine locations were conducted from 2011 to 2015 in southern and central Brazil to characterize the efficacy of DAS-81419-2 soybean infested with Anticarsia gemmatalis Hübner (Lepidoptera: Erebidae), Chrysodeixis includens (Walker) (Lepidoptera: Noctuidae), Heliothis virescens (F.) (Lepidoptera: Noctuidae), and Spodoptera cosmioides (Walker) (Lepidoptera: Noctuidae) during vegetative (V4) and reproductive (R2 and R4) crop developmental stages. The efficacy of DAS-81419-2 was compared to that of a non-Bt isogenic variety managed with or without applications of commercial foliar insecticides for lepidopteran control. DAS-81419-2 soybean consistently experienced defoliation levels of 0.5% or less (compared with 20.05-56.74% in the non-Bt, nonsprayed treatment) and larval survival of < 0.1% in all four species across the vegetative and reproductive plant stages evaluated. The efficacy of DAS-81419-2 was significantly higher than commercial foliar insecticides applied to the non-Bt variety. DAS-81419-2 soybeans containing two highly effective Bt proteins are expected to be a more robust IRM tool compared to single-trait Bt technologies. The consistent efficacy of DAS-81419-2 soybeans across years, locations, and crop stages suggests that it will be a valuable product for management of hard-to-control key lepidopteran pests in South American soybean production.

Keywords: Anticarsia gemmatalis; Chrysodeixis includens; Conkesta; Heliothis virescens; Spodoptera cosmioides.

MeSH terms

  • Animals
  • Bacillus thuringiensis / genetics
  • Bacillus thuringiensis Toxins
  • Bacterial Proteins / pharmacology*
  • Brazil
  • Endotoxins / pharmacology*
  • Glycine max / genetics*
  • Hemolysin Proteins / pharmacology*
  • Insect Control / methods
  • Insecticides / pharmacology
  • Larva / drug effects
  • Larva / growth & development
  • Moths / drug effects*
  • Moths / growth & development
  • Pest Control, Biological*
  • Plants, Genetically Modified / genetics*
  • Species Specificity

Substances

  • Bacillus thuringiensis Toxins
  • Bacterial Proteins
  • Endotoxins
  • Hemolysin Proteins
  • Insecticides
  • insecticidal crystal protein, Bacillus Thuringiensis