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BMC Complement Altern Med. 2016 Jul 11;16:202. doi: 10.1186/s12906-016-1192-z.

Rubus occidentalis alleviates hyperalgesia induced by repeated intramuscular injection of acidic saline in rats.

Author information

1
Department of Anesthesiology and Pain Medicine, College of Medicine, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul, 06911, Republic of Korea.
2
Department of Anesthesiology and Pain Medicine, College of Medicine, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul, 06911, Republic of Korea. roman00@naver.com.
3
Department of Anesthesiology and Pain Medicine, Ewha Womans University School of Medicine, Seoul, Korea.
4
Gochang Berry & Bio Food Research Institute, Gochang-gun, Jeollabuk-do, Korea.

Abstract

BACKGROUND:

The purpose of this study was to evaluate the antinociceptive effect of black raspberry (Rubus occidentalis) fruit extract (ROE) in a rat model of chronic muscle pain and examine the mechanisms involved.

METHODS:

Adult male Sprague-Dawley rats were used, and chronic muscle pain was induced by two injections of acidic saline into one gastrocnemius muscle. For the first experiment, 50 rats were randomly assigned to five groups. After the development of hyperalgesia, rats were injected intraperitoneally with 0.9 % saline or ROE (10, 30, 100, or 300 mg/kg). For the second experiment, 70 rats were randomly assigned to seven groups. Rats were injected intraperitoneally with saline, yohimbine, dexmedetomidine, prazosin, atropine, mecamylamine, or naloxone after the development of hyperalgesia. Ten minutes later, ROE (300 mg/kg) was administered intraperitoneally. For both experiments, the mechanical withdrawal threshold (MWT) was evaluated with von Frey filaments before the first acidic saline injection, 24 h after the second injection, and at 15, 30, 45, 60, 80, 100, and 120 min, 24 and 48 h after the drug administration.

RESULTS:

Compared with the control group, the MWT significantly increased up to 45 min after injection of ROE 100 mg/kg and up to 60 min after injection of ROE 300 mg/kg, respectively. Injection of ROE together with yohimbine or mecamylamine significantly decreased the MWT compared with the effect of ROE alone, while ROE together with dexmedetomidine significantly increased the MWT.

CONCLUSIONS:

ROE showed antinociceptive activity against induced chronic muscle pain, which may be mediated by α2-adrenergic and nicotinic cholinergic receptors.

KEYWORDS:

Antinociception; Black raspberry; Chronic pain; Rubus occidentalis

PMID:
27400712
PMCID:
PMC4940828
DOI:
10.1186/s12906-016-1192-z
[Indexed for MEDLINE]
Free PMC Article

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