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ACS Appl Mater Interfaces. 2016 Jul 20;8(28):17878-84. doi: 10.1021/acsami.6b05471. Epub 2016 Jul 11.

Long-Term Tracking of the Osteogenic Differentiation of Mouse BMSCs by Aggregation-Induced Emission Nanoparticles.

Author information

1
State Key Laboratory of Luminescent Materials and Devices, South China University of Technology , Guangzhou 510640, China.
2
National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology , Guangzhou 510640, China.
3
Department of Chemistry, The Hong Kong University of Science & Technology , Clear Water Bay, Kowloon, Hong Kong, China.
4
Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction , Hong Kong, China.

Abstract

Bone marrow-derived mesenchymal stem cells (BMSCs) have shown great potential for bone repair due to their strong proliferation ability and osteogenic capacity. To evaluate and improve the stem cell-based therapy, long-term tracking of stem cell differentiation into bone-forming osteoblasts is required. However, conventional fluorescent trackers such as fluorescent proteins, quantum dots, and fluorophores with aggregation-caused quenching (ACQ) characteristics have intrinsic limitations of possible interference with stem cell differentiation, heavy metal cytotoxicity, and self-quenching at a high labeling intensity. Herein, we developed aggregation-induced emission nanoparticles decorated with the Tat peptide (AIE-Tat NPs) for long-term tracking of the osteogenic differentiation of mouse BMSCs without interference of cell viability and differentiation ability. Compared with the ability of the commercial Qtracker 655 for tracking of only 6 passages of mouse BMSCs, AIE-Tat NPs have shown a much superior performance in long-term tracking for over 12 passages. Moreover, long-term tracking of the osteogenic differentiation process of mouse BMSCs was successfully conducted on the biocompatible hydroxyapatite scaffold, which is widely used in bone tissue engineering. Thus, AIE-Tat NPs have promising applications in tracking stem cell fate for bone repair.

KEYWORDS:

aggregation-induced emission; bone repair; long-term cell tracking; osteogenic differentiation; stem cell

PMID:
27400339
DOI:
10.1021/acsami.6b05471
[Indexed for MEDLINE]

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