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Am J Transl Res. 2016 Jun 15;8(6):2659-66. eCollection 2016.

Effects of 1,25(OH)2D3 on proliferation and apoptosis of human glomerular mesangial cells.

Author information

1
Department of Nephrology, The first Affiliated Hospital, School of Medicine, Shihezi University Xinjiang 832000, China.
2
Medical College of Shihezi University Shihezi, Xinjiang 832000, China.

Abstract

OBJECTIVE:

To investigate the effect of 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] on cell proliferation and apoptosis of human glomerular mesangial cells.

METHODS:

Human glomerular mesangial cells were cultured for 48 h and randomly divided into four groups, normal control group (N group), proliferation group (EGF group ), vitamin D3 intervention group (VD3 group), proliferation intervention group (EGF+VD3 group ). Flow cytometry was used to detect the cell cycle and apoptosis rate, and Western blot was used to detect the PCNA and Caspases-3 expression. The effect of 1,25(OH)2D3 on human mesangial cell proliferation was detected by CCK-8.

RESULTS:

Compared with the control group, G1 phase cells in EGF group were significantly reduced, S, G2/M phase cells were increased, and the higher PI and PCNA expression levels were increased; G1 phase cells in VD3 group were increased, S, G2/M phase cells were decreased, and the lower PI and PCNA expression levels were reduced. Compared with EGF group, G1 phase cells in EGF+VD3 group were increased, S, G2/M phase cells were decreased, and the lower PI and PCNA expression levels were significantly reduced. Compared with normal control group, the apoptosis rate in EGF group was significantly lower, and the Caspase-3 expression level was reduced; the apoptosis rate in VD3 group was significantly higher, and Caspase-3 expression was significantly increased. Compared with EGF group, the apoptotic rate of mesangial cells in EGF+VD3 group was significantly increased, and the Caspase-3 expression level was increased, with significant difference.

CONCLUSION:

1,25(OH)2D3 inhibited the proliferation of mesangial cells and induced their apoptosis by blocking the cell cycle, inhibiting expression of PCNA, and upregulating Caspase-3 expression.

KEYWORDS:

1; 25(OH)2D3]; 25-dihydroxyvitamin D3 [1; Caspase-3; Mesangial cells; epidermal growth factor (EGF); proliferating cell nuclear antigen (PCNA)

PMID:
27398149
PMCID:
PMC4931160

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