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Mol Cell. 2016 Jul 21;63(2):240-248. doi: 10.1016/j.molcel.2016.05.040. Epub 2016 Jul 7.

Redox Nanodomains Are Induced by and Control Calcium Signaling at the ER-Mitochondrial Interface.

Author information

1
MitoCare Center, Department of Pathology, Anatomy and Cell Biology, Thomas Jefferson University, Philadelphia, PA 19107, USA.
2
Department of Physiology, Faculty of Medicine, Semmelweis University, 1444 Budapest, Hungary.
3
MitoCare Center, Department of Pathology, Anatomy and Cell Biology, Thomas Jefferson University, Philadelphia, PA 19107, USA. Electronic address: gyorgy.hajnoczky@jefferson.edu.

Abstract

The ER-mitochondrial interface is central to calcium signaling, organellar dynamics, and lipid biosynthesis. The ER and mitochondrial membranes also host sources and targets of reactive oxygen species (ROS), but their local dynamics and relevance remained elusive since measurement and perturbation of ROS at the organellar interface has proven difficult. Employing drug-inducible synthetic ER-mitochondrial linkers, we overcame this problem and demonstrate that the ER-mitochondrial interface hosts a nanodomain of H2O2, which is induced by cytoplasmic [Ca(2+)] spikes and exerts a positive feedback on calcium oscillations. H2O2 nanodomains originate from the mitochondrial cristae, which are compressed upon calcium signal propagation to the mitochondria, likely due to Ca(2+)-induced K(+) and concomitant water influx to the matrix. Thus, ER-mitochondrial H2O2 nanodomains represent a component of inter-organelle communication, regulating calcium signaling and mitochondrial activities.

PMID:
27397688
PMCID:
PMC4998968
DOI:
10.1016/j.molcel.2016.05.040
[Indexed for MEDLINE]
Free PMC Article

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