Format

Send to

Choose Destination
PLoS One. 2016 Jul 7;11(7):e0158944. doi: 10.1371/journal.pone.0158944. eCollection 2016.

Next-Generation Sequencing of an 88-Year-Old Specimen of the Poorly Known Species Liagora japonica (Nemaliales, Rhodophyta) Supports the Recognition of Otohimella gen. nov.

Author information

1
Kobe University Research Center for Inland Sea, Awaji, Hyogo, Japan.
2
Center for Life Science Research, University of Yamanashi, Yamanashi, Japan.
3
National Institute of Polar Research, Midori-cho, Tachikawa-shi, Tokyo, Japan.
4
Transdisciplinary Research Integration Center, Toranomon, Minato-ku, Tokyo, Japan.
5
Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Meguro-ku, Tokyo, Japan.
6
Department of Natural Sciences, Faculty of Arts and Sciences, Komazawa University, Setagaya-ku, Tokyo, Japan.
7
Faculty of Chemical and Biological Sciences, Japan Women's University, Tokyo, Japan.
8
Bioresource Sciences, Faculty of Agriculture, Kyushu University, Higashi-ku, Fukuoka, Japan.
9
Department of Biology, Graduate School of Science, Kobe University, Rokkodai, Nada-ku, Kobe, Japan.
10
Department of Botany, National Museum of Nature and Science, Tsukuba, Ibaraki, Japan.
11
The Hokkaido University Museum, Hokkaido University, Sapporo, Hokkaido, Japan.
12
Department of Biological Sciences, Faculty of Science, Hokkaido University, Sapporo, Hokkaido, Japan.
13
Department of Biological Sciences, Graduate School of Science, University of Tokyo, Hongo, Bunkyo-ku, Tokyo, Japan.

Abstract

Liagora japonica is a red algal species distributed in temperate regions of Japan. This species has not been collected from its type locality on the Pacific coast of Japan since 1927 and seems to have become extinct in this area. For molecular characterization of L. japonica, we extracted DNA from the topotype material of L. japonica collected in 1927, analyzed seven genes using Illumina next-generation sequencing, and compared these data with sequences from modern samples of similar red algae collected from the Japan Sea coast of Japan. Both morphological and molecular data from modern samples and historical specimens (including the lectotype and topotype) suggest that the specimens from the Pacific and Japan Sea coasts of Japan should be treated as a single species, and that L. japonica is phylogenetically separated from the genus Liagora. Based on the phylogenetic results and examination of reproductive structures, we propose Otohimella japonica gen. et comb. nov., characterized morphologically by diffuse carposporophytes, undivided carposporangia, and involucral filaments initiated only from the cortical cell on the supporting cell.

PMID:
27388436
PMCID:
PMC4936710
DOI:
10.1371/journal.pone.0158944
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Public Library of Science Icon for PubMed Central
Loading ...
Support Center