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Sci Rep. 2016 Jul 7;6:29565. doi: 10.1038/srep29565.

Quantifying mammalian genomic DNA hydroxymethylcytosine content using solid-state nanopores.

Author information

1
Virginia Tech-Wake Forest University School of Biomedical Engineering and Sciences, Wake Forest University School of Medicine, Winston-Salem, NC 27101, USA.
2
Department of Chemistry, Department of Biochemistry and Molecule Biology, Institute for Biophysical Dynamics, The University of Chicago, 929 East 57th Street, Chicago, Illinois 60637, USA.
3
Howard Hughes Medical Institute, The University of Chicago, 929 East 57th Street, Chicago, Illinois 60637, USA.
4
Comprehensive Cancer Center, Wake Forest University School of Medicine, Winston-Salem, NC 27101, USA.

Abstract

5-hydroxymethylcytosine (5 hmC), the oxidized form of 5-methylcytosine (5 mC), is a base modification with emerging importance in biology and disease. However, like most epigenetic elements, it is transparent to many conventional genetic techniques and is thus challenging to probe. Here, we report a rapid solid-state nanopore assay that is capable of resolving 5 hmC with high specificity and sensitivity and demonstrate its utility in assessing global modification abundance in genomic DNA.

PMID:
27383905
PMCID:
PMC4935868
DOI:
10.1038/srep29565
[Indexed for MEDLINE]
Free PMC Article

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