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J Appl Oral Sci. 2016 May-Jun;24(3):278-90. doi: 10.1590/1678-775720150481.

TNF-alpha expression, evaluation of collagen, and TUNEL of Matricaria recutita L. extract and triamcinolone on oral ulcer in diabetic rats.

Author information

1
- Universidade Federal do Ceará, Setor de Patologia Oral, Departamento de Odontologia Clínica, Fortaleza, CE, Brasil.
2
- Universidade Federal do Ceará, Setor de Cariologia e Odontologia Restauradora, Departamento de Odontologia Restauradora, Fortaleza, CE, Brasil.
3
- Universidade Estadual do Ceará, Setor de Fisiologia e Farmacologia, Departamento de Ciências Biomédicas, Fortaleza, CE, Brasil.
4
- Universidade Federal do Ceará, Departamento de Fisiologia e Farmacologia, Fortaleza, CE, Brasil.

Abstract

OBJECTIVE:

to evaluate the influence of Tumor Necrosis Factor alpha (TNF-α) and apoptosis in rats with DM treated with chamomile extract or triamcinolone.

MATERIAL AND METHODS:

Wistar male rats (210.0±4.2 g) were divided into five groups: negative control group (NCG) without diabetes; positive control group (PCG) with DM (alloxan, 45 mg/kg); and groups treated with chamomile extract (normoglycemic= NCG group and diabetic= DCG group) and with triamcinolone (TG). Traumatic ulcers were performed on all animals that received topical triamcinolone, chamomile extract or saline 12/12 hours for ten days.

RESULTS:

On days five and ten the animals were euthanized and the ulcers were analyzed by light microscopy, TUNEL assay, and immunohistochemically (TNF-α). The NCG (p=0.0062), PCG (p=0.0285), NCG (p=0.0041), and DCG (p<0.0001) groups were completely healed on the 10th day, however, there was no healing on the TG (p=0.5127) group. The TNF-α expression showed a significant reduction from the 5th to the 10th day in NCG (p=0.0266) and DCG (p=0.0062). In connective tissue, the TUNEL assay showed a significant reduction in the number of positive cells in NCG (p=0.0273) and CNG (p=0.0469) and in the epithelium only in CDG (p=0.0320).

CONCLUSIONS:

Chamomile extract can optimize the healing of traumatic oral ulcers in diabetic rats through the reduction of apoptosis in the epithelium and TNF-α expression.

PMID:
27383710
PMCID:
PMC5022216
DOI:
10.1590/1678-775720150481
[Indexed for MEDLINE]
Free PMC Article

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