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Nucleic Acids Res. 2016 Nov 2;44(19):9462-9471. Epub 2016 Jul 4.

Inverted repeat Alu elements in the human lincRNA-p21 adopt a conserved secondary structure that regulates RNA function.

Author information

1
Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06511, USA.
2
Howard Hughes Medical Institute, Chevy Chase, MD 20815, USA.
3
Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06511, USA anna.pyle@yale.edu.
4
Department of Chemistry, Yale University, New Haven, CT 06511, USA.

Abstract

LincRNA-p21 is a long intergenic non-coding RNA (lincRNA) involved in the p53-mediated stress response. We sequenced the human lincRNA-p21 (hLincRNA-p21) and found that it has a single exon that includes inverted repeat Alu elements (IRAlus). Sense and antisense Alu elements fold independently of one another into a secondary structure that is conserved in lincRNA-p21 among primates. Moreover, the structures formed by IRAlus are involved in the localization of hLincRNA-p21 in the nucleus, where hLincRNA-p21 colocalizes with paraspeckles. Our results underscore the importance of IRAlus structures for the function of hLincRNA-p21 during the stress response.

PMID:
27378782
PMCID:
PMC5100600
DOI:
10.1093/nar/gkw599
[Indexed for MEDLINE]
Free PMC Article

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