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Comp Biochem Physiol Part D Genomics Proteomics. 2016 Sep;19:78-89. doi: 10.1016/j.cbd.2016.06.005. Epub 2016 Jun 17.

Understanding mechanism of sea cucumber Apostichopus japonicus aestivation: Insights from TMT-based proteomic study.

Author information

1
Fisheries College, Ocean University of China, Qingdao, PR China. Electronic address: chenmuyan@gmail.com.
2
Fisheries College, Ocean University of China, Qingdao, PR China.
3
Institute of Biochemistry, Carleton University, 1125 Colonel By Drive, Ottawa, ON K1S 5B6, Canada.
4
Institute of Oceanology, Chinese Academy of Sciences, Qindao, PR China.
5
Fishery College, Zhejiang Ocean University, Zhoushan, PR China.
6
Marine Science and Technology College, Zhejiang Ocean University, Zhoushan, PR China.

Abstract

Marine invertebrate aestivation is a unique strategy for summer survival in response to hot marine conditions. The sea cucumber, Apostichopus japonicus, is an excellent model marine invertebrate for studies of environmentally-induced aestivation. In the present study, we used a tandem mass tag (TMT)-coupled LC-MS/MS approach to identify and quantify the global proteome expression profile over the aestivation-arousal cycle of A. japonicus. A total of 3920 proteins were identified from the intestine of sea cucumber. Among them, 630 proteins showed significant differential expression when comparing three conditions of sea cucumbers: non-aestivating (active), deep-aestivation (at least 15days of continuous aestivation), and arousal after aestivation (renewed moving and feeding). Sea cucumbers in deep aestivation showed substantial differentially expressed proteins (143 up-regulated and 267 down-regulated proteins compared with non-aestivating controls). These differentially expressed proteins suggested that protein and phospholipid probably are major fuel sources during hypometabolism and a general attenuation of carbohydrate metabolism was observed during deep aestivation. Differentially expressed proteins also provided the first global picture of a shift in protein synthesis, protein folding, DNA binding, apoptosis, cellular transport and signaling, and cytoskeletal proteins during deep aestivation in sea cucumbers. A comparison of arousal from aestivation with deep aestivation, revealed a general reversal of the changes that occurred in aestivation for most proteins. Western blot detection further validated the significant up-regulation of HSP70 and down-regulation of methyltransferase-like protein 7A-like in deep-aestivation. Our results suggest that there is substantial post-transcriptional regulation of proteins during the aestivation-arousal cycle in sea cucumbers.

KEYWORDS:

Aestivation; Post-transcriptional regulation; Proteome; Sea cucumber; Tandem mass tag

PMID:
27376927
DOI:
10.1016/j.cbd.2016.06.005
[Indexed for MEDLINE]

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