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Ecotoxicol Environ Saf. 2016 Oct;132:390-6. doi: 10.1016/j.ecoenv.2016.06.025. Epub 2016 Jul 1.

The ceramide inhibitor fumonisin B1 mitigates the pulmonary effects of low-dose diesel exhaust inhalation in mice.

Author information

1
The Center for Environmental Health Science for the Next Generation, Research Institute for Science and Technology, Organization for Research Advancement, Tokyo University of Science, Japan; Department of Pharmacology, Faculty of Veterinary Medicine, Damanhour University, Egypt. Electronic address: dr_hazemshaheen3010@yahoo.com.
2
The Center for Environmental Health Science for the Next Generation, Research Institute for Science and Technology, Organization for Research Advancement, Tokyo University of Science, Japan; Department of Hygienic Chemistry, Graduate School of Pharmaceutical Sciences, Tokyo University of Science, Japan; Research Fellow of Japan Society for the Promotion of Science, Japan. Electronic address: atsutoyukari@gmail.com.
3
The Center for Environmental Health Science for the Next Generation, Research Institute for Science and Technology, Organization for Research Advancement, Tokyo University of Science, Japan. Electronic address: shinkai49@gmail.com.
4
Department of Hygienic Chemistry, Graduate School of Pharmaceutical Sciences, Tokyo University of Science, Japan. Electronic address: m.n.canalcatfish@gmail.com.
5
The Center for Environmental Health Science for the Next Generation, Research Institute for Science and Technology, Organization for Research Advancement, Tokyo University of Science, Japan; Department of Pharmacology, Faculty of Veterinary Medicine, South Valley University, Egypt. Electronic address: ashraf_elghoneimy@yahoo.com.
6
Department of Veterinary Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Damanhour University, Egypt. Electronic address: yasser_tf@yahoo.com.
7
The Center for Environmental Health Science for the Next Generation, Research Institute for Science and Technology, Organization for Research Advancement, Tokyo University of Science, Japan. Electronic address: takedak@rs.noda.tus.ac.jp.
8
The Center for Environmental Health Science for the Next Generation, Research Institute for Science and Technology, Organization for Research Advancement, Tokyo University of Science, Japan. Electronic address: masa-ume@rs.noda.tus.ac.jp.

Abstract

Recent studies have suggested that inhalation of diesel exhaust (DE), a major source of air pollution, results in pulmonary alterations; however, the effects of DE at low concentrations are poorly understood. Therefore, this study was conducted to elucidate the pulmonary effects of low-level exposure to DE and the potential role of a ceramide de novo biosynthesis inhibitor, fumonisin B1 (FB1) to ameliorate the DE-toxicity. Male C57BL/6J mice underwent 1- or 7-day experiments (4 equal groups/experiment) and were assigned to the control, DE (0.1mg/m(3)), FB1 (6.75mg/kg body weight SC at days 0, 3 and 6) or DE+FB1 groups. DE and/or FB1 treatment had no effect on the expression of Nos2, a biomarker of oxidative stress. Ceramide production in the bronchial epithelial cells and Sphk1 mRNA expression were induced in the lung after the 7-day DE exposure and were partially suppressed by the FB1 treatment. Additionally, the effects of DE on SP-A and SP-D mRNA expression were also suppressed by the FB1 treatment. These results suggest that ceramide and Sphk1 may be sensitive biomarkers for low-level DE-induced pulmonary effects. Collectively, ceramide likely contributes to the DE-induced early stage of airway inflammation, which is considered a potential pulmonary target during low-level DE exposure.

KEYWORDS:

Ceramide; Diesel exhaust; Fumonisin B1; Immunohistochemistry; Lung; RT-qPCR

PMID:
27376354
DOI:
10.1016/j.ecoenv.2016.06.025
[Indexed for MEDLINE]

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