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Biomed Opt Express. 2016 May 24;7(6):2362-72. doi: 10.1364/BOE.7.002362. eCollection 2016 Jun 1.

Combination of an optical parametric oscillator and quantum-dots 655 to improve imaging depth of vasculature by intravital multicolor two-photon microscopy.

Author information

1
Institut des Neurosciences de la Timone, Marseille, Aix-Marseille Université and CNRS UMR7289, France; Centre Européen de Recherche en Imagerie Médicale, Aix-Marseille Université, Marseille, France; Service de Pharmacie, Assistance Publique, Hôpitaux de Marseille, Marseille, France; Centre de Recherche en Oncobiologie et Oncopharmacologie, INSERM UMR911 and Aix-Marseille Université, Marseille, France; These authors contributed equally to this work.
2
Institut des Neurosciences de la Timone, Marseille, Aix-Marseille Université and CNRS UMR7289, France; Centre Européen de Recherche en Imagerie Médicale, Aix-Marseille Université, Marseille, France; These authors contributed equally to this work.
3
Institut des Neurosciences de la Timone, Marseille, Aix-Marseille Université and CNRS UMR7289, France; Centre Européen de Recherche en Imagerie Médicale, Aix-Marseille Université, Marseille, France.

Abstract

Simultaneous imaging of different cell types and structures in the mouse central nervous system (CNS) by intravital two-photon microscopy requires the characterization of fluorophores and advances in approaches to visualize them. We describe the use of a two-photon infrared illumination generated by an optical parametric oscillator (OPO) on quantum-dots 655 (QD655) nanocrystals to improve resolution of the vasculature deeper in the mouse brain both in healthy and pathological conditions. Moreover, QD655 signal can be unmixed from the DsRed2, CFP, EGFP and EYFP fluorescent proteins, which enhances the panel of multi-parametric correlative investigations both in the cortex and the spinal cord.

KEYWORDS:

(170.0170) Medical optics and biotechnology; (170.3880) Medical and biological imaging

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