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Food Chem. 2016 Dec 1;212:537-42. doi: 10.1016/j.foodchem.2016.06.014. Epub 2016 Jun 7.

Protective effects of ID331 Triticum monococcum gliadin on in vitro models of the intestinal epithelium.

Author information

1
Istituto di Scienze dell'Alimentazione, Consiglio Nazionale delle Ricerche, via Roma, 64, 83100 Avellino, Italy.
2
Istituto di Scienze dell'Alimentazione, Consiglio Nazionale delle Ricerche, via Roma, 64, 83100 Avellino, Italy; Dipartimento di Agraria, Università Federico II, Parco Gussone, 80055 Portici (Na), Italy.
3
Consiglio per la Ricerca in Agricoltura e l'analisi dell'economia Agraria, Unità di Ricerca per la Valorizzazione Qualitativa dei Cereali (CRA-QCE), via Manziana, 30, 00189 Roma, Italy.
4
Istituto di Scienze dell'Alimentazione, Consiglio Nazionale delle Ricerche, via Roma, 64, 83100 Avellino, Italy. Electronic address: mamone@isa.cnr.it.

Abstract

A growing interest in developing new strategies for preventing coeliac disease has motivated efforts to identify cereals with null or reduced toxicity. In the current study, we investigate the biological effects of ID331 Triticum monococcum gliadin-derived peptides in human Caco-2 intestinal epithelial cells. Triticum aestivum gliadin derived peptides were employed as a positive control. The effects on epithelial permeability, zonulin release, viability, and cytoskeleton reorganization were investigated. Our findings confirmed that ID331 gliadin did not enhance permeability and did not induce zonulin release, cytotoxicity or cytoskeleton reorganization of Caco-2 cell monolayers. We also demonstrated that ID331 ω-gliadin and its derived peptide ω(105-123) exerted a protective action, mitigating the injury of Triticum aestivum gliadin on cell viability and cytoskeleton reorganization. These results may represent a new opportunity for the future development of innovative strategies to reduce gluten toxicity in the diet of patients with gluten intolerance.

KEYWORDS:

Caco-2; Coeliac disease; Cytotoxicity; F-actin; Triticum monococcum; Zonulin; ω-Gliadin

PMID:
27374565
DOI:
10.1016/j.foodchem.2016.06.014
[Indexed for MEDLINE]

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