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J Biotechnol. 2016 Sep 10;233:74-83. doi: 10.1016/j.jbiotec.2016.06.011. Epub 2016 Jun 30.

Versatility of chemically synthesized guide RNAs for CRISPR-Cas9 genome editing.

Author information

1
Dharmacon, part of GE Healthcare, Lafayette, CO, 80026, USA.
2
Dharmacon, part of GE Healthcare, Lafayette, CO, 80026, USA. Electronic address: anja.smith@ge.com.

Abstract

The CRISPR-Cas9 system has become the most popular and efficient method for genome engineering in mammalian cells. The Streptococcus pyogenes Cas9 nuclease can function with two types of guide RNAs: the native dual crRNA and tracrRNA (crRNA:tracrRNA) or a chimeric single guide RNA (sgRNA). Although sgRNAs expressed from a DNA vector are predominant in the literature, guide RNAs can be rapidly generated by chemical synthesis and provide equivalent functionality in gene editing experiments. This review highlights the attributes and advantages of chemically synthesized guide RNAs including the incorporation of chemical modifications to enhance gene editing efficiencies in certain applications. The use of synthetic guide RNAs is also uniquely suited to genome-scale high throughput arrayed screening, particularly when using complex phenotypic assays for functional genomics studies. Finally, the use of synthetic guide RNAs along with DNA-free sources of Cas9 (mRNA or protein) allows for transient CRISPR-Cas9 presence in the cell, thereby resulting in a decreased probability of off-target events.

KEYWORDS:

Dual RNA; Gene editing; RNA synthesis; Synthetic crRNA; Synthetic tracrRNA

PMID:
27374403
DOI:
10.1016/j.jbiotec.2016.06.011
[Indexed for MEDLINE]
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