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Curr Biol. 2016 Jul 25;26(14):1802-15. doi: 10.1016/j.cub.2016.05.035. Epub 2016 Jun 30.

Neural Mechanisms Generating Orientation Selectivity in the Retina.

Author information

1
MRC Centre for Developmental Neurobiology, King's College London, Guy's Campus, London SE1 1UL, UK.
2
MRC Centre for Developmental Neurobiology, King's College London, Guy's Campus, London SE1 1UL, UK. Electronic address: robert.hindges@kcl.ac.uk.

Abstract

The orientation of visual stimuli is a salient feature of visual scenes. In vertebrates, the first neural processing steps generating orientation selectivity take place in the retina. Here, we dissect an orientation-selective circuit in the larval zebrafish retina and describe its underlying synaptic, cellular, and molecular mechanisms. We genetically identify a class of amacrine cells (ACs) with elongated dendritic arbors that show orientation tuning. Both selective optogenetic ablation of ACs marked by the cell-adhesion molecule Teneurin-3 (Tenm3) and pharmacological interference with their function demonstrate that these cells are critical components for orientation selectivity in retinal ganglion cells (RGCs) by being a source of tuned GABAergic inhibition. Moreover, our morphological analyses reveal that Tenm3(+) ACs and orientation-selective RGCs co-stratify their dendrites in the inner plexiform layer, and that Tenm3(+) ACs require Tenm3 to acquire their correct dendritic stratification. Finally, we show that orientation tuning is present also among bipolar cell presynaptic terminals. Our results define a neural circuit underlying orientation selectivity in the vertebrate retina and characterize cellular and molecular requirements for its assembly.

PMID:
27374343
PMCID:
PMC4963213
DOI:
10.1016/j.cub.2016.05.035
[Indexed for MEDLINE]
Free PMC Article

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