Format

Send to

Choose Destination
BMC Genet. 2016 Jul 2;17(1):100. doi: 10.1186/s12863-016-0408-z.

Is premeiotic genome elimination an exclusive mechanism for hemiclonal reproduction in hybrid males of the genus Pelophylax?

Author information

1
Laboratory of Fish Genetics, Department of Vertebrate Evolutionary Biology and Genetics, Institute of Animal Physiology and Genetics CAS v.v.i, Liběchov, 277 21, Czech Republic. dolezalkova@iapg.cas.cz.
2
Department of Zoology, Faculty of Science, Charles University in Prague, Praha 2, 128 43, Czech Republic. dolezalkova@iapg.cas.cz.
3
Laboratory of Fish Genetics, Department of Vertebrate Evolutionary Biology and Genetics, Institute of Animal Physiology and Genetics CAS v.v.i, Liběchov, 277 21, Czech Republic.
4
Department of Genetics and Microbiology, Faculty of Science, Charles University in Prague, Viničná 5, Prague 2, 128 44, Czech Republic.
5
Laboratory of Molecular Cytogenetics, Institute of Entomology, Biology Centre CAS, České Budějovice, 370 05, Czech Republic.
6
Museum für Naturkunde, Leibniz Institute for Evolution and Biodiversity Science, Invalidenstraße 43, Berlin, 10115, Germany.
7
Department of Biology and Ecology, Faculty of Science, University of Ostrava, Chittussiho 10, Ostrava, 710 00, Czech Republic.

Abstract

BACKGROUND:

The ability to eliminate a parental genome from a eukaryotic germ cell is a phenomenon observed mostly in hybrid organisms displaying an alternative propagation to sexual reproduction. For most taxa, the underlying cellular pathways and timing of the elimination process is only poorly understood. In the water frog hybrid Pelophylax esculentus (parental taxa are P. ridibundus and P. lessonae) the only described mechanism assumes that one parental genome is excluded from the germline during metamorphosis and prior to meiosis, while only second genome enters meiosis after endoreduplication. Our study of hybrids from a P. ridibundus-P. esculentus-male populations known for its production of more types of gametes shows that hybridogenetic mechanism of genome elimination is not uniform.

RESULTS:

Using comparative genomic hybridization (CGH) on mitotic and meiotic cell stages, we identified at least two pathways of meiotic mechanisms. One type of Pelophylax esculentus males provides supporting evidence of a premeiotic elimination of one parental genome. In several other males we record the presence of both parental genomes in the late phases of meiotic prophase I (diplotene) and metaphase I.

CONCLUSION:

Some P. esculentus males have no genome elimination from the germ line prior to meiosis. Considering previous cytological and experimental evidence for a formation of both ridibundus and lessonae sperm within a single P. esculentus individual, we propose a hypothesis that genome elimination from the germline can either be postponed to the meiotic stages or absent altogether in these hybrids.

KEYWORDS:

Asexual propagation; Genomic in situ hybridization; Hemiclone; Hybridogenesis; Meiotic cycle; Rana esculenta

PMID:
27368375
PMCID:
PMC4930623
DOI:
10.1186/s12863-016-0408-z
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for BioMed Central Icon for PubMed Central
Loading ...
Support Center