Format

Send to

Choose Destination
Iran J Cancer Prev. 2016 Feb 22;9(1):e3919. doi: 10.17795/ijcp-3919. eCollection 2016 Feb.

Autophagy Gene Activity May Act As a Key Factor for Sensitivity of Tumor Cells to Oncolytic Vesicular Stomatitis Virus.

Author information

1
Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IR Iran.
2
Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, IR Iran.
3
Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IR Iran; Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, IR Iran.

Abstract

BACKGROUND:

Beclin1 is an important, primary molecule for autophagy.

OBJECTIVES:

It is suggested that the control of the autophagy path increases the sensitivity of tumor cells to VSV.

MATERIALS AND METHODS:

In this study, the degree of Beclin1 gene expression in two cell lines, HeLa and A549, has been examined and the percentage of living cells subsequent infection with virus has been evaluated by MTT assay method.

RESULTS:

The results showed that the degree of Beclin1 gene expression in HeLa cells in comparison with A549 cells has reduced, and the sensitivity of these cells to vesicular stomatits virus (VSV) oncolysis is more than A549.

CONCLUSIONS:

It seems that by using some methods for reducing autophagy, it is possible to make tumor cells more sensitive to virotherapy and even other treatments.

KEYWORDS:

Autophagy; Beclin1; Vesicular Stomatitis Virus; Virotherapy

Supplemental Content

Full text links

Icon for PubMed Central
Loading ...
Support Center